3130xl Fragment Analysis - Microsatellites and T-RFLPs
Microsatellite markers, also known as short tandem repeats (STRs), are polymorphic DNA loci consisting of a repeated nucleotide sequence. The number of repeats varies in a population, thus creating multiple alleles for a microsatellite locus. Microsatellite loci are amplified by PCR using fluorescently labeled forward primer. These PCR amplicons are then separated by size on the AB 3130xl genetic analyzer. T-RFLP analysis is a technique used to study complex microbial communities based on variation in the 16S rRNA gene. It can be used to study microbial community structure, bacterial populations in the environment and changes in a microbial community in response to different environmental parameters.
Our instrument runs batches of 16 samples - but entire plates can be set-up and scheduled to run on the instrument.
Please submit a Job Request Form, see link below, with your samples and specify the size standard you want us to use. This is a downloadable Excel file that you will fill out and submit electronically to the MRCF, as well as send a hard copy along with the samples submitted to us.
First time customers, will need to submit a one-time New User Form.
Available Size Standards: ROX350, LIZ600, ROX1000
Please send a minimum of 5ml of sample. For a new fragment analysis project, we recommend that several stock dilutions be run on the sequencer to determine the optimal sample dilution, for example 1:10, 1:100, 1:200.
$2.00 / sample
DNA/RNA Profiling by Advance Analytical's Fragment Analyzer
The Fragment Analyzer covers the full spectrum of DNA, gDNA and RNA quality control. It is used and recommended by Illumina as an automated system for the quantification and qualification of NGS libraries, gDNA and RNA. Our instrument has 12-capillaries that electrophoreses 11-fluorescently labeled samples and a size standard (ladder) per instrument run. With a run time of a little less than 1 hour, it resolves fragments from 10-40,000 bp. The instrument has a resolution of 2bp for fragments which can be detected in as little concentration of 5 pg/uL. The instrument is one of the best to look at the profile, integrity and quality of samples.
$2.50 / sample (internal and external)