Skip to main content

Technology in Lab

General Troubleshooting

  • Computers are not letting you past the opening OS screen:
    1. restart;
    2. if restart does not work, replace computer with one of the towers in the back of 262;
    3. put problem tower on instructor bench with a note and then send an email to the coordinator.
  • Projector does not display:
    1. Make sure the projector and computer are connected. Find the cable and dongle that connects the two. Jiggle them a bit; sometimes they look connected but a few of the pins are not in contact.  OR
    2. Press:
      WinP
    3. Then select 'Duplicate': 
      Screen

Vernier Troubleshooting

  • Computer not recognizing Vernier Logger Pro or Vernier sensors: 
    1. unplug Go!Link from computer,
    2. unplug sensor from Go!Link,
    3. restart (not just Logging off) computer.
  • Logger Pro/Sensor not taking readings:
    1. close (not just minimize) the Logger Pro software,
    2. unplug Go!Link from computer,
    3. unplug sensor from Go!Link,
    4. open Logger Pro cmbl file needed for the exercise.
    5. plug  in Go!Link to the computer,
    6. plug in the sensor to the Go!Link,
  • Vernier sensor readings seem inaccurate: calibrate.

Venier equipment is used in several lab exercises. The Logger Pro software and the Go!Link interface are used in conjuction with several sensors:

   pH    |   Conductivity   |   Gas Pressure  |   CO2   |    O2

pH Sensor

A. Use

  • The storage buffer solution must completely cover the sensor tip when on its side or upside down. Keep the storage vial filled to the top.
  • The sensor must be rinsed and blotted dry between uses.
  • The sensor has a glass tip; it can break.
  • The pH sensors do not need to warm-up. They should instantly read 4 because the storage buffer is at pH 4.
    1. Connect the Go!Link to your computer using the USB cable.
    2. Using Logger Pro, open the specific .cmbl file needed for the exercise.
    3. If the initial reading is under or over 4, skip to section B below.

B. Calibration

If you find that the initial reading is under or over 4 when you first plug in the sensor and open Logger Pro, then follow the procedure below.

  1. Go to the Tool Bar and select ‘Experiment’ > ‘Calibration’ > ‘Go!Link: pH.
  2. The ‘Calibrate’ tab will be open in the window that pops up.
  3. Follow the 2-point calibration procedure:
    1. Remove the sensor from the storage vial by unscrewing the cap. Remove the vial but slide the cap up the shaft of the sensor.
    2. Place sensor in buffer standard 4
    3. Click on ‘Keep’.
    4. Rinse the tip with a wash bottle and blot dry with a Kimwipe.
    5. Place sensor in buffer standard 7
    6. Click on ‘Keep’.
    7. Rinse the tip with a wash bottle and blot dry with a Kimwipe.
    8. Place the sensor back in the storage vial that is filled with storage buffer.
  4. Once the calibration is complete click on ‘Done’. The ‘Current Calibration:’ drop down will show ‘pH <Not Saved>’
  5. Go back to the Tool Bar and select ‘Experiment’ > ‘Calibration’ > ‘Go!Link: pH’.
  6. Once the window opens select the ‘Calibration Storage’ tab.
  7. Follow the calibration storage procedure:
    1. Make sure that the ‘Current Calibration:’ drop down shows ‘pH <Not Saved>’.
    2. Click on ‘Set Sensor Calibration’. A new window will open.
    3. Click on ‘Set’, ‘Done’, ‘Write’.
  8. Unplug the pH sensor from the Go!Link and then plug it in again. If the Logger Pro reading window does not read around 4 the calibration storage procedure did not work. Repeat section B. If the initial reading is under or over 4

Conductivity Sensor

A. Use 

Set switch on conductivity sensors to μS/cm range required for the lab exercise.

  • Low range 0 to 200 μS/cm (0 to 100 mg/L TDS)
  • Mid range 0 to 2000 μS/cm (0 to 1000 mg/L TDS)
  • High range 0 to 20,000 μS/cm (0 to 10,000 mg/L TDS)

B. Calibration

Calibration should not be required.

 Gas Pressure Sensor 

A. Use 

The white stem on the end of the Gas Pressure Sensor has a small threaded end called a luer lock. With a gentle half turn, you may attach the plastic tubing to this stem using one of the Luer connectors already mounted on both ends of the tubing. The Luer connector at the other end of the plastic tubing can then be connected to one of the stems on the rubber stoppers.   The stopper can then be inserted into a flask or test  tube to provide an airtight container to investigate a confined gas.

B. Calibration

Calibration should not be required.  

CO2 Sensor

A. Use

  • Make sure the switch on the sensor is set to LOW 0 - 10,000ppm.
  • The CO2 sensors need to warm-up for 45 seconds to 2 minutes. They are ready to use once they read 400 ±100 ppm and the reading is somewhat stable (doesn’t jump around too much).
    1. Connect the Go!Link to your computer using the USB cable.
    2. Place the CO2 gas sensor upright in a dry beaker and then plug it into the Go!Link.
    3. Using Logger Pro, open the specific .cmbl file needed for the exercise.
    4. Wait between 45 seconds to 2 minutes for the reading to reach 400 ±100 ppm.
    5. If the reading is under 300 or over 500, skip to section B below.
  • Between runs keep the sensor sitting upright in a dry beaker.
  • Do not blow on the sensor shaft.
  • Do not get the sensor wet. It is meant to be used only with air.

 B. Calibration

The sensors were checked to make sure they read 400 ±100 ppm. However, if you find that after you have completed steps 1-4 from section A, the readings are under 300 OR over 500 go through the calibration procedure.

  1. Place the sensor upright in a dry beaker.
  2. Plug the sensor into the Go!Link.
  3. Open Logger Pro.
  4. Wait 2 minutes to allow the sensor to warm-up.
  5. After 2 minutes, hold the sensor calibration button down (found on side of sensor) until the spot below the button flashes a red/orange light
  6. After about 30 seconds, the reading should stabilize at a value of approximately 400 ±100 ppm. If the reading is under 300 OR over 500, simply press the button again to repeat the process. Note: If the red light stays on solid, you have held the button too long. In this case, wait for it to go off and press it again, releasing as soon as it begins to flash.
  7. Close and Re-open Logger Pro.
  8. Wait 2 minutes and then check that the calibration worked and the reading is still 400 ±100 ppm.

O2 Sensor 

A. Use

  • Keep upright at ALL times.
  • Place in a reaction chamber ONLY during a run.
  • Between runs keep the sensor sitting upright in a DRY BEAKER.
  • Do not get the sensor wet. It is meant to be used only with air.
  • Do not blow on the sensor shaft.
  • The O2 sensors do not need to warm-up. They should instantly read 20.9 ±1.0%.
    1. Connect the Go!Link to your computer using the USB cable.
    2. Place the CO2 gas sensor upright in a dry beaker and then plug it into the Go!Link.
    3. Using Logger Pro, open the specific .cmbl file needed for the exercise.
    4. If the initial reading is under 19.0% or Over 22%, skip to section B below.

B. Calibration

The sensors were checked to make sure they were in the 0-20.9% O2 gas range. However, if you find that the reading is significantly lower than 20.9% when you first plug in the sensor and open Logger Pro, then follow the procedure below.

  1. Go to the Tool Bar and select ‘Experiment’ > ‘Calibration’ > ‘Go!Link: 1 Oxygen Gas’.
  2. The ‘Calibrate’ tab will be open in the window that pops up.
  3. Follow the 2-point calibration procedure:
    1. While holding the calibration button down (found on side of sensor) enter a value of 0.
    2. Click on ‘Keep’.
    3. Release the calibration button.
    4. Enter a value of 20.9% oxygen.
    5. Click on ‘Keep’.
    6. The Logger Pro reading window should show the calibration.
  4. Once the calibration is complete click on ‘Done’. The ‘Current Calibration:’ drop down will show ‘Oxygen Gas % <Not Saved>’
  5. Go back to the Tool Bar and select ‘Experiment’ > ‘Calibration’ > ‘Go!Link: 1 Oxygen Gas’.
  6. Once the window opens select the ‘Calibration Storage’ tab.
  7. Follow the calibration storage procedure:
    1. Make sure that the ‘Current Calibration:’ drop down shows ‘Oxygen Gas % <Not Saved>’.
    2. Click on ‘Set Sensor Calibration’. A new window will open.
    3. Click on ‘Set’, ‘Done’, ‘Write’.
  8. Unplug the O2 sensor from the Go!Link and then plug it in again. If the Logger Pro reading window does not read around 20.9%, the calibration storage procedure did not work. Repeat section B.
 

Remove the plastic protective cover and turn on the spectrophotometer using the power switch located on the back of the machine, lower left side.  The spectrophotometer will automatically perform some diagnostics and will need to warm up for 30 minutes before measurements can be made. Once it has warmed up, it should automatically be set to absorbance (A) mode.  If it is not, press the A/T/C button to select absorbance (A).

  1. Measure your blank by adding a blank solution to the cuvette (or test tube). Wipe down the outside of the test tube with ethanol and a kimwipe to remove fingerprints.
  2. Insert your cuvette into the cell holder of the sample chamber. The cuvette should be sitting on the bottom of the cell.
  3. Close the sample chamber.
  4. Press the ABS button to set the blank to 0 concentration.
  5. Select the wavelength you will be measuring your solutions at by press the nm buttons.
  6. Measure your samples by inserting the cuvette into the cell holder.  Remember to clean the outside of the cuvette before placing into the cell holder. Do not get fingerprints on the cuvette as it will disrupt the reading.
  7. Read your absorbance.
  8. Remove your sample and replace it with a new sample and record the absorbance.

At the end of the lab period (if there isn’t a lab immediately following), shut off the machine and replace the plastic cover.

If you receive an error message use the Operator's Manual for Genesys 20 Spectrophotometer.

The AVer Document Camera and AVer (Sphere) Software are used for dissections and presentations.

Microscopes are used by the students in many lab exercises. Instructors also need to learn to use the LAS EZ & Leica AirLab software which will allow them to project the microscope images in real time.

IDAHO STATE UNIVERSITY

921 South 8th Avenue
Pocatello, Idaho, 83209

Discover opportunity at Idaho State University