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Multiphoton (2P) Microscope, Olympus FV100MPE
The multiphoton microscope excitation is superior for imaging living cells that reside within intact tissues such as brain slices, embryos, whole organs, and even entire animals. The technique provides optical sectioning with reduced absorption of excitation light in specimen regions removed from the objective focal plane, thus minimizing photobleaching and phototoxicity. Please talk to MRCF staff to see if this system is right for you!
Confocal Microscope, Olympus FV1000
Our confocal laser scanning microscope has four simultaneous detection channels, including one for DIC and two spectral channels. The lasers used for excitation on our instrument cover the visible spectrum; the exact wavelengths are 405nm, 458nm, 488nm, 515nm, 559nm and 635nm. It is important to talk to one of our staff members before setting up an experiment to make sure there won't be crosstalk or crossing of two spectrally-similar colors.
Leica DM6B Complete Upright Microscope
The DM6B is a fully automated microscope capable of brightfiled, darkfield, DIC, phase contrast, and epifluorescence imaging. The DM6B can also simultaneously capture fluorescence and DIC, and fluorescence and phase images. The ease of use makes this microscope ideal for teaching courses, and great for quick sample screening.
Transmission Electron Microscopy
Zeiss EM900 TEM
The Transmission Electron Microscope (TEM) is capable of imaging ultra-thin specimen at the magnification of 400,000X, - easily detecting targets as small as cellular organelles and viruses. Use of the TEM requires meticulous sample preparation of tissue support mesh grids coated in a layer of carbon molecules. Our newly acquired Gatan CCD digital camera makes image capture quick and easy. Sample preparation is specialized for this instrument, please contact MRCF staff with questions about this process.
Histology Equipment and Offline Data Analysis Station
Cryostat, Leica 3050 S
When working with delicate specimens the precise specimen orientation and the specimen feed system of the Leica cryostat guarantees reproducible, thin, serial sections of maximum quality. The 3050 S is the top of the line model for cryostats, and is capable of automatic, foot-pedal driven sectioning for convenience of the user. A second cryostat, the Leica 3050, is also available for use.
Microtome, Leica RM2255
Designed for fully motorized sectioning of both paraffin- and resin-embedded specimens, the Leica RM2255 supports a broad spectrum of applications.
Offline Data Analysis Station
This offline workstation includes multiple programs to help researchers analyze data generated from the Advanced Imaging Facility, and produce publication worthy images and pictures for various uses. Programs available on this computer are MetaMorph for Olympus, ImageJ (Fiji), Vaa3D - 3D Visualization-Assisted Analysis, Volocity Demo 6.1.1 and FluoRender. In addition to analysis software, the computer includes Microsoft Office and Adobe Reader.