Flow Cytometry

Example data from the flow cytometer from BD Biosciences

Flow Cytometry is the analysis of fluorescent markers and dyes on virtually any cell or cell-sized particle. It is a simple, rapid and powerful tool that takes advantage of fluidics, laser and PMT technology, and high tech electronics for detection and analysis of fluorescence.

The FACSCalibur incorporates efficient optics and two air-cooled solid-state lasers to analyze up to four colors. Compact and easy to use, the FACSCalibur is a highly sensitive tool, providing researchers with the technological capabilities crucial for sophisticated cellular and molecular work in areas such as cell cycle, cell function, multicolor analysis, and immune function.

Our instrument has a mechanical sorter that allows researchers to sort out cell populations of interest, and save them for other applications. The sorter is able to sort at around 2,500 cells/second, which is fast enough to collect large numbers of cells in a fairly short time, and keep them viable and ready to use.

 

 

Cost

Currently, there is no charge for using the Flow Cytometer. We have excess reagents and a fully funded service contract. We encourage our researchers to take advantage of this while it lasts!

Read this brief Tech Note on how to use Flow Cytometry to quantify the amount of living cells versus dead cells in a sample.

 

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