Template DNA

Starting with the correct concentration of template DNA is critical to getting good sequencing data. Below is the nanogram amount of DNA needed for each sequencing reaction based on the type of DNA you are sequencing.

All samples need to be diluted in water to a total volume of 5uL.

Table of DNA concentrations for Sanger Sequencing

 

 

Universal Primers Available

We provide the following universal primers for DNA sequencing.

If you would like us to add these to your samples, it must be specified on the Job Request Form.

List of primers provided by MRCF

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