Molecular Research Core Facility

Next-Generation Sequencing

Please contact Erin O'Leary-Jepsen directly, oleaerin@isu.edu, for a quote on specific NGS projects.

Price List

Dehalococcoides spp. Detection

Detect and quantify Dehalococcoides spp. and the reductase genes bvcA, tceA, and vcrA in environmental samples for bioremediation projects.
Please contact MRCF staff for rates and additional information.

DNA Sequencing

$5.00/sample
If you submit full (95 samples) or half (47 samples) plates with the template and primer mixed together at the required concentration. This will allow for one pGem control sample per full or half plate. Samples over one half plate in other than groups of 16 will be charged the $7.00/sample rate. Samples must be submitted in 96-well plates or 8-well strip tubes.
$6.00/sample
If you submit samples in groups of 16, as long as they are in plates or 8-well strip tubes with the template and primer mixed together at the required concentration. Samples in other than groups of 16 will be charged the $7.00/sample rate.
$7.00/sample
If you submit fewer than 16 samples with the template and primer mixed together at the required concentration (0.5 ml tubes, 1.5 ml tubes, 8-well strip tubes, or plates). Due to the 16-capillary format of our sequencer, we may have to wait and batch your samples with those from another researcher.

Adjustments may be considered based on specific handling of a particular project.

Fragment Analysis

$2.00/sample
If you submit samples in groups of 16, as long as they are in plates or 8-well strip tubes. Includes size standard and processing on the 3130XL. Samples in other than groups of 16 will be charged the $2.50/sample rate.
$2.50/sample
If you submit fewer than 16 samples (0.5 ml tubes, 1.5 ml tubes, 8-well strip tubes, or plates). Includes size standard and processing on the 3130XL. Due to the 16-capillary format of our sequencer, we may have to wait and batch your samples with those from another researcher.

DNA sequencing services include sample preparation (cycle sequencing) using Applied Biosystems' Big Dye Terminator chemistry, post cycle sequencing sample purification, and sample processing on our Applied Biosystems 3130XL Genetic Analyzer. After processing we upload data to our Galaxy data management server, where you can view, download, and print the sequence information and/or original chromatograms. After processing you will receive an e-mail message with instructions for downloading your data from Galaxy. A copy of Sequencher DNA sequencing analysis software is available for your use on a Macintosh computer kiosk in the MRCF.

Fragment analysis services include post PCR sample preparation using the appropriate size standard for your samples, and sample processing on our Applied Biosystems 3130XL Genetic Analyzer. After processing we upload your data to our Galaxy data management server, where you can view, download, and print your data files. After processing you will receive an e-mail message with instructions for downloading your data from Galaxy. A copy of GeneMapper software for data analysis is available for your use on a PC computer kiosk in the MRCF.

COST STRUCTURE FOR USAGE OF MRCF SHARED INSTRUMENTATION
Fees provide service contract support and help to cover depreciation costs.

CFX96 $20.00/run; During project optimization phase consult with manager about discounted rate.
Qubit 2.0 Fluorometer Please contact MRCF staff for usage costs and additional information.
NanoDrop $0.15/sample internal ($0.25/sample if you are an external researcher requesting quantification of your samples for sequencing)
Bio-Rad VersaDoc $0.25/minute
MJ Thermal Cycler $10.00/run; During project optimization phase consult with manager about discounted rate.
MJ Chromo4 $20.00/run; During project optimization phase consult with manager about discounted rate.
Jouan MR22i $5.00/hour
Sorvall Legend RT $5.00/hour
Nu-Aire Clean Bench $2.00/hour
Hemco Fume Hood $2.00/hour
Nanopure water $0.25/liter
Bio-Rad VersaDoc prints $0.10/print
HP LaserJet prints $0.10/print
Tri-Carb Liquid Scintillation Counter $0.25/sample for first 20 samples, then $0.10/sample

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